Question

A cloning vector does not have a selectable marker. How will it affect the process of cloning?

Answer 1

As it is challenging to differentiate between cells that have incorporated the vector with the desired DNA insert and those that have not, the lack of a selectable marker in a cloning vector makes the process more challenging. Selectable indicators, such as genes for antibiotic resistance, aid in the development and selection of transformed cells, making the procedure simpler by enabling the isolation of only the cells that have effectively incorporated the vector.