Question

Enlist the basic steps involved in recombinant DNA technology

Answer 1

1) Isolating genomic DNA of a ‘donor’. The cell or organism from which the required gene is taken is called 'donor'.

2) Fragmenting this DNA using “molecular scissors” (Restriction endonuclease Enzymes).

3) Screening the fragments for a ‘desired gene’.

4) Inserting the fragments with the desired gene into a ‘cloning vector’. (a plasmid, cosmid, or phage DNA) so as to develop a recombinant DNA or chimeric DNA.

5) Introducing the recombinant vector into a competent host cell

6) Culturing these cells to obtain multiple copies or clones of the desired fragment of DNA

7) Using these copies to “Transform” suitable host cells so as to express the desired gene