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प्रश्न
Explain the role of the Selectable markers in pBR322 in Biotechnology.
उत्तर
pBR322 has two antibiotic resistance genes, one for ampicillin and other one for tetracycline. Antibiotic resistance serves as selectable marker. If the foreign DNA is ligated at the site of tetracycline resistance gene in pBR322 vector, the recombinant plasmid will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from non-recombinants by plating the transformants on ampicillin containing medium. The transformants growing on ampicillin containing medium are then transferred on a medium containing tetracycline. The recombinants will grow in ampicillin containing medium but not on the tetracycline-containing medium. However the non recombinants will grow on both. Thus by using antibiotic resistant genes as selectable markers, we can differentiate between recombinants and non-recombinants.
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