Advertisements
Advertisements
प्रश्न
Gene expression can be controlled with the help of RNA. Explain the method with an example.
उत्तर
Gene expression can be controlled by using RNA molecules. The technology is called RNA interference or RNAi. It is used to block the expression of certain genes and also referred to as gene silencing. During this process, a complementary RNA to the mRNA being produced by the gene is introduced into the cell. This RNA binds to the mRNA making it double-stranded and therefore stops translation. Resistance to nematode Meloidogyne incognita in tomato has been achieved by this method.
APPEARS IN
संबंधित प्रश्न
what is golden rice.
List any five possible advantages of a GMO to a farmer.
Answer the following question:
Why do farmers find the production of hybrid seeds costly?
Answer the following question.
List any four ways by which GMOs have been useful for enhanced crop output.
GM crops especially Bt crops are known to have higher resistance to pest attacks. To substantiate this an experimental study was conducted in 4 different farmlands growing Bt and non Bt-Cotton crops. The farm lands had the same dimensions, fertility and were under similar climatic conditions. The histogram below shows the usage of pesticides on Bt crops and non-Bt crops in these farm lands.
- Which of the above 4 farm lands has successfully applied the concepts of Biotechnology to show better management practices and use of agrochemicals? If you had to cultivate, which crop would you prefer (Bt or Non- Bt) and why?
- Cotton Bollworms were introduced in another experimental study on the above farm lands wherein no pesticide was used. Explain what effect would a Bt and Non Bt crop have on the pest.
A person deficient in the rhodopsin should be advised to take more of:
Bt cotton is not ______.
Discuss the advantages of GMO.
Explain with the help of one example how genetically modified plants can:
- Reduce usage of chemical pesticides
- Enhance nutritional value of food crops
|
RNA interference (RNAi) holds great potential as a therapeutic agent for the treatment of human diseases and as biocontrol agents in controlling pests in the field agriculture. An experiment was carried to study the use of 'RNAi' for the potential treatment of disorders of cholesterol metabolism. Some people possess genetic mutations with elevated levels of ApoB gene which predisposes them to coronary artery diseases. Lowering the amount ApoB can reduce the number of lipoproteins and lower the blood cholesterol. Tracy Zimmerman and her colleagues used RNAi in 2006 to reduce the level of ApoB in non human primates Cynomolgus monkeys. One group of monkeys were given RNAi treatment (small interfering RNAs, SiRNAs) (doses 1 mg/kg, SiRNAs), second group of monkeys were given RNAi treatment (doses 2.5 mg/kg, SiRNAs) and third group of monkeys were injected with saline. |
The results of the study are depicted in the graph below:
- How does the treatment with 2.5 mg/kg bring an effect on cholesterol metabolism when compared from 24 hours and 144 hours?
- Write any two natural sources from where dsRNA molecule could be obtained for silencing the specific mRNA.
- How is RNAi used in controlling the infection on the roots of tobacco plants by the nematode Meloidogyne incognitia?
