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प्रश्न
Give a reason why :
Single cloning site is preferred in a vector.
उत्तर
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Do eukaryotic cells have restriction endonucleases? Justify your answer.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?
