Advertisements
Advertisements
प्रश्न
How has the use of Agrobacterium as vectors helped in controlling Meloidegyne incognitia infestation in tobacco plants? Explain in correct sequence.
उत्तर
The following steps were taken utilizing Agrobacterium as a transforming vector for tobacco plants to make them immune against the infestation of a nematode Meloidegyne incognitia:
1) Suitable plasmid vectors are chosen and the specific nematode gene is inserted into it.
2) The recombinant plasmids are introduced into the competent Agrobacterium cells.
3) The transformed Agrobacterium cells are screened and selected to infect the tobacco plants.
4) Agrobacterium infection on the tobacco plant cultures leads to insertion and integration of nematode-desired DNA fragment within the tobacco plant.
5) Correct insertion of nematode gene into the host tobacco plants leads to silencing of a essential housekeeping gene of the Meloidegyne incognitia pest by RNAi.
APPEARS IN
संबंधित प्रश्न
Find out what the various components of the medium used for propagation of an explants in vitro are?
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because −
What are biofertilizers? Describe their role in agriculture. Why are they preferred to chemical fertilizers?
A person deficient in the rhodopsin should be advised to take more of:
A cell swells up when kept in:
At what temperature milk gets pasteurised?
Assertion (A): In Bt cotton, B. thuringiensis produces a toxic insecticidal crystalline protein which destroys bollworms.
Reason (R): B. thuringiensis produces this toxic protein in an inactive form, but when an insect ingests this inactive protein, it is converted into active form of toxin due to the alkaline pH of gut which solubilises the crystals, which is responsible for the death of bollworm.
Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produces (in the host cells).
You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.
The main objective of the production of pest-resistant GM crops is to ______.
