Question

How will you avoid the growing of microbes in the nutrient medium during the culture process? What are the techniques used to remove the microbes?

Answer 1

The microbial growth in the culture medium can be overcome by autoclaving the medium at Plant Tissue Culture II 121°C (15 psi) for 15 to 30 minutes.

Chemical sterilization using chemicals, sterilizing using UV radiation. Alcoholic sterilization using ethanol, autoclaving and filtration, etc., are the various techniques used to remove microbes.