Question

Identify the process in Column I with the term in Column II and select the correct option.

Column I	Column II
A. Amplification of gene of interest	I. Downstream processing
B. Large-scale production of plants in a short duration.	II. Polymerase chain reaction
C. Separation and purification of the desired product	III. Transformation
D. Insertion of rDNA into the host	IV. Micropropagation

विकल्प

• A - IV, B - II, C - I, D - III

• A - II, B - IV, C - III, D - I

• A - II, B - IV, C - I, D - III

• A - II, B - I, C - IV, D - III

Answer 1

A - II, B - IV, C - I, D - III

Explanation:

• Polymerase chain reaction, or PCR, is used to amplify the gene of interest. It is performed in vitro and is used to generate billions of copies of desired genes.
• Plant micropropagation is an effective method for growing large numbers of disease-resistant plants in vitro. The entire technique is carried out under temperature and light intensity controls.
• Downstream processing refers to the isolation and purification of the intended gene or product. It is primarily employed in large-scale metabolite synthesis.
• Transformation refers to the process of transferring rDNA into the host cell. Prior to the procedure, the bacterial cells are made competent through various methods such as electroporation.