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प्रश्न
'Restriction' in Restriction enzyme refers to ______.
विकल्प
Cleaving of phosphodiester bond in DNA by the enzyme
Cutting of DNA at specific position only
Prevention of the multiplication of bacteriophage by the host bacteria
All of the above
उत्तर
'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Restriction enzymes ______.
'Restriction' in restriction enzyme refers to
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?