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प्रश्न
What is the cause of discontinuous synthesis of DNA on one of the parental strands of DNA? What happens to these short stretches of synthesised DNA?
उत्तर
The DNA-dependent DNA polymerases catalyse polymerisation only in one direction, that is 5′ → 3′. This creates some additional complications at the replicating fork. Consequently, on leading strand (the template with polarity 3′→ 5′), the replication is continuous, while on the lagging strain (the template with polarity 5′ → 3′), it is discontinuous. The discontinuously synthesised fragments are later joined by the enzyme DNA ligase.
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संबंधित प्रश्न
Discontinuous synthesis of DNA occurs in one strand, because ______.
An enzyme that joins the ends of two strands of nucleic acid is a ______.
Other than DNA polymerase, which are the enzymes involved in DNA synthesis?
Read the following and answer from given below:
The process of copying genetic information from the template strand of DNA into RNA is called transcription. It is mediated by RNA polymerase. Transcription takes place in the nucleus of eukaryotic cells. In transcription, only a segment of DNA and only one of the strands is copied into RNA.
Which enzyme helps in tailing or polyadenylation?
Feedback inhibition of enzymes is affected by ______.
The discontinuously synthesized fragments are joined by the enzyme ______.
The enzyme DNA polymerase in E.coli is a DNA dependent polymerase and also has the ability to proof-read the DNA strand being synthesised. Explain. Discuss the dual polymerase.
In a nucleus, the number of ribonucleoside triphosphates is 10 times the number of deoxy x10 ribonucleoside triphosphates, but only deoxy ribonucleotides are added during the DNA replication. Suggest a mechanism.
Name a few enzymes involved in DNA replication other than DNA polymerase and ligase. Name the key functions for each of them.
During DNA replication, why is it that the entire molecule does not open in one go? Explain replication fork. What are the two functions that the monomers (dNTPs) play?
