Question

With the help of a neat and labelled diagram describe the steps in recombinant DNA technology.

Answer 1

Steps in recombinant DNA technology:-

The following steps are involved in recombinant DNA technology:-

• Isolation of genomic DNA from the donor.
• Fragmentation of the isolated DNA fragment by using restriction enzymes.
• Screening of the fragments for the desired gene.
• Insertion of the fragments with the desired gene into a cloning vector so as to develop a recombinant or chimeric DNA.
• Introduction of the recombinant vector into a competent host cell.
• Culturing of the cells containing recombinant vectors to obtain multiple copies of the desired fragment of DNA.
• Use of these copies to transform suitable host cells so as to express the desired gene.