Question

A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.

पर्याय

• b, a, c, a

• a, b, c, d

• c, b, a, d

• b, a, d, c

Answer 1

b, a, c, a

Explanation:

The relative molecular weight of these DNA fragments determines their position on the electrophoresis.

The fragment with the lowest molecular weight will appear further away from the wall, while the fragment with the highest molecular weight will appear closest to the wall. Since the molecular weight of d is the highest and the molecular weight of b is the lowest, the order of the fragments will be as follows: (b, a, c, a ).