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प्रश्न
Answer the following questions based on Hershey and Chases's experiments:
(a) Name the kind of virus they worked with and why?
(b) Why did they use two types of culture media to grow viruses in ? Explain.
(c) What was the need for using a blender and later a centrifuge during their experiments?
(d) State the conclusion drawn by them after the experiments.
How did Hershey and Chase established that DNA is transferred from virus to bacteria?
उत्तर १
a) Hershey and Chase worked on bacteriophages because they have the ability to infect bacteria. When a bacteriophage infects a bacterium, the viral genetic material gets attached with the bacterial genetic material and the bacterium then treats the viral genetic material as its own to synthesise more viral particles.
(b) They grew some phages in a medium with radioactive sulphur, while others in a medium with radioactive phosphorus. The reason behind this was to label DNA and proteins separately in order to indentify which one is acting as genetic material.
(c) Then, they blended E. coli and centrifuged it to remove viral particles and then checked presence of radioactivity.
(d) They observed that the bacteria with radioactive DNA were radioactive, while those with radioactive proteins had lost their radioactivity.
This showed that it is the DNA that enters the bacteria from viruses and not proteins. Hence, it was concluded that DNA is the genetic material.
उत्तर २
Hershey and Chase worked on bacteriophages (viruses that infect bacteria).
When a bacteriophage infects a bacterium, the viral genetic material gets attached with the bacterial genetic material and then, bacteria treats the viral genetic material as its own to synthesise more viral particles.
Hershey and Chase worked to discover whether it was a protein or DNA that entered the bacteria from virus.
They labelled some phages with radioactive sulphur and the others with radioactive phosphorus.
These radioactive phages were used to infect E. coli.
Then, E.coli was blended and centrifuged to remove viral particles.
It was observed that bacteria with radioactive DNA were radioactive while those with radioactive proteins lost their radioactivity.
This showed that it is the DNA that enters the bacteria from viruses and not proteins. Hence, it was concluded that DNA is the genetic material.
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संबंधित प्रश्न
Give reasons for the following:
The genetic code is 'universal'
Describe the experiment performed by Griffith. What conclusions did he infer from his observations?
Describe Avery, McLeod and McCarty’s experiment. State its significance.
Hershey and Chase experiment with bacteriophage showed that ____________.
The two strands of DNA are held together by ______.
Which one is a hereditary disease?
The transforming principle of Pneumococcus as found out by Avery, MacLeod, and McCarty was ______.
Which of the following methods of reproduction in bacteria involves participation of a virus?
Name the scientist who proved experimentally that DNA is the genetic material ______.
Define transformation in Griffith's experiment. Discuss how it helps in the identification of DNA as the genetic material.
