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प्रश्न
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
उत्तर
The palindromic sequences i.e. the sequence of base pairs read the same on both the DNA strands when the orientation of reading is kept the same, eg :
5' —— GAATTC —— 3'
3' —— CTTAAG —— 5'
- Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Give a reason why :
Single cloning site is preferred in a vector.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
'Restriction' in Restriction enzyme refers to ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following bacteria is not a source of restriction endonuclease?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How are DNA fragments visualised once they are separated by gel electrophoresis?
