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Explain the concept of tissue culture. - Science and Technology

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प्रश्न

Explain the concept of tissue culture.

थोडक्यात उत्तर

उत्तर

  1. ‘Ex vivo growth of cells or tissues in an aseptic and nutrient-rich medium’ is called tissue culture.
  2. Nowadays, a complete organism can be developed from a single cell or from tissue with help of the tissue culture technique.
  3. A liquid, solid or gel-like medium prepared from agar, which supplies nutrients and energy necessary for tissue culture is used in this technique.
  4. Its uses are in the production of cash crops, improvement in varieties of cash crops, increase in abilities of plants to withstand environmental stresses, vaccine production, early diagnosis of congenital diseases, organ transplant, cancer research, production of artificial skin, cartilage, etc. in laboratories.


                    Various processes in tissue-culture

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पाठ 17: Introduction to Biotechnology - Exercise [पृष्ठ २०८]

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बालभारती Science and Technology [English] 9 Standard Maharashtra State Board
पाठ 17 Introduction to Biotechnology
Exercise | Q 9. ii | पृष्ठ २०८

संबंधित प्रश्‍न

What is 'tissue culture'?


Why are the plants raised through micropropagation are termed as somaclones?


With advancements in genetics, molecular biology and tissue culture, new traits have been incorporated into crop plants. Explain the main steps in breeding a new genetic variety of a crop


Explain micropropagation and somatic hybridization.


Mention one significant difference between Symplastic movement and apoplastic movement


Give three anatomical differences between a monocot root and a dicot root


Explain the transpiration pull theory for an ascent of sap


You have obtained a high yielding variety of tomato. Name and explain the procedure that ensures retention of the desired characteristics repeatedly in large populations of future generations of the tomato crop.


How can healthy potato plants be obtained from a desired potato variety which is viral infected? Explain.

 

 

List any four applications of tissue culture. 


To get a disease-free plant from a virus infected plant, which one of the following can be used as an explant in tissue culture?


Which of the following helps bamboo and grasses to elongate?


Given below are a few statements regarding somatic hybridization. Choose the correct statements.

  1. protoplasts of different cells of the same plant are fused
  2. protoplasts from cells of different species can be fused
  3. treatment of cells with cellulase and pectinase is mandatory
  4. the hybrid protoplast contains characters of only one parental protoplast

An explant is ______.


Would it be wrong to call plants obtained through micro-propagation as ‘clones’? Comment.


What are the physical barriers of a cell in the protoplast fusion experiment? How are the barriers overcome?


Write a short note on callus culture.


Write a short note on suspension culture.


What does the plant tissue culture medium consists of?


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