Advertisements
Advertisements
प्रश्न
Explain the principle of paper chromatography.
उत्तर
In paper chromatography, chromatography paper is used. This paper contains water trapped in it, which acts as the stationary phase. On the base of this chromatography paper, the solution of the mixture is spotted. The paper strip is then suspended in a suitable solvent, which acts as the mobile phase. This solvent rises up the chromatography paper by capillary action and in the procedure, it flows over the spot. The components are selectively retained on the paper (according to their differing partition in these two phases). The spots of different components travel with the mobile phase to different heights. The paper so obtained (shown in the given figure) is known as a chromatogram.
APPEARS IN
संबंधित प्रश्न
Which separation technique will you apply for the separation of the following?
Different pigments from an extract of flower petals.
The best and latest technique for isolation, purification and separation of organic compounds is ______.
What are the advantages of chromatography?
Name the simplest type of chromatography?
On what principle is this method based?
Name the different types of chromatographic techniques.
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Label the diagram and explain the process in your words.
Chromatography is based on the principle of ______.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
The principle involved in paper chromatography is ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?