Question

How has the use of Agrobacterium as vector helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in correct sequence.

Answer 1

The use of Agrobacterium as a vector has aided in the management of the nematode Meloidogyne incognita infestation in tobacco plants via genetic engineering. Here's the order of events:

1. Using Agrobacterium vectors, nematode-specific genes were delivered into host plants.
2. The introduction of DNA resulted in the production of both sense and antisense RNA in host cells. These two complementary RNAs created a double-stranded (dsRNA) that began RNA interference (RNAi) and thereby silenced the nematode's particular mRNA.
3. As a result, the parasite was unable to live in a transgenic host that expressed particular interfering RNA. As a result, the transgenic plant became parasite-resistant.