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प्रश्न
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
उत्तर
- Restriction endonucleases: for cutting the desired DNA at desired places.
- Gel electrophoresis: for separating the desired DNA fragments.
- Ligase enzyme: for creating recombinant DNA molecule.
- DNA delivery system: like electroporation, microinjection, gene gun method.
- Competent host (usually bacteria/yeast): to take up recombinant DNA.
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संबंधित प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
What is genetically engineered Insulin?
The site of production of ADA the body is ______.
ELISA is based on:
Taking examples under each category, discuss upstream and downstream processing.
How is a mature, functional insulin hormone different from its prohormone form?
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
Name the technique used to detect the presence of HIV in the body of an individual, Justify the principle associated with this technique.
