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महाराष्ट्र राज्य शिक्षण मंडळएचएससी विज्ञान (सामान्य) इयत्ता १२ वी

What is the role of Taq-polymerase in PCR technology? - Biology

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प्रश्न

What is the role of Taq-polymerase in PCR technology?

एका वाक्यात उत्तर

उत्तर

Taq-polymerase helps in polymerization of DNA molecules in PCR technology.

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पाठ 12: Biotechnology - Very Short Answer

संबंधित प्रश्‍न

One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.

 

 

Alu-I is obtained from which organism?


Match the List I with List II and List ill. Select the correct option.

  List I   List II   List ill
i. Hind III a. Agarose ge 1. Six base pairs
ii. pBR322 b. Agrobacterium 2. Selectable marke
iii. T-DNA c. Ampicillin 3. Elution
iv. DNA d. Recognition sequenc 4. Transgenic  plant

Which of the following DNA sequence is recognised and cut by EcoR I?


Match the column I (PCR steps) and Column II (Temperature). Select the correct option.

  Column I (PCR steps)   Column II (Temperature)
i. Polymerization P. 40 - 60°C
ii. Denaturation Q. 70 - 75°C
iii. Primer annealing R. 90 - 98°C
    S. 28 - 37°C

A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______


Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil.

(a) List two advantages of using bacteria for such biotechnological studies?

(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.

(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.


Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?


The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.


Which one of the following enzymes is used to join DNA fragments? 


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