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प्रश्न
What is the use of southern blotting in DNA fingerprinting?
उत्तर
In the Southern blotting technique, the DNA fragments separated using agarose gel electrophoresis are transferred to a nylon membrane or a nitrocellulose filter paper by placing it over the gel and soaking them with filter paper overnight.
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संबंधित प्रश्न
Briefly describe the following:
Polymorphism
How is repetitive/satellite DNA separated from bulk genomic DNA for various genetic experiments?
What are the different types of mutations?
Which of the following technique is used for amplification or multiplication of DNA for fingerprinting?
The process in which sample DNA and probe DNA form double stranded DNA is called ____________.
Indole-3-acetic acid (auxin) was first isolated from ______.
Polymorphism arises due to ______
The technique of DNA fingerprinting was first given by ______.
Match the following pairs:
| Column I | Column II | ||
| (i) | PCR | (a) | DNA transferred to nylon membrane |
| (ii) | Electrophoresis | (b) | DNA amplification |
| (iii) | Southern blotting | (c) | Restriction digestion |
| (iv) | Autoradiography | (d) | Produce fragments of different size |
| (v) | Alkali treatment | (e) | Separation of DNA fragments on gel slab |
| (vi) | DNA treated with REN | (f) | X-ray photography |
Enlist the steps in DNA fingerprinting giving its applications.
