Question

Write about Pachytene and Diplotene of Prophase I

Answer 1

1. Pachytene: At this stage, bivalent chromosomes are clearly visible as tetrads. Bivalent of meiosis I consist of 4 chromatids and 2 centromeres. Synapsis is completed and recombination nodules appear at a site where crossing over takes place between non – sister chromatids of the homologous chromosomes. Recombination of homologous chromosomes is completed by the end of the stage but the chromosomes are linked at the sites of crossing over. This is mediated by the enzyme recombinase.
2. Diplotene: Synaptonemal complex disassembled and dissolves. The homologous chromosomes remain attached at one or more points where crossing over has taken place. These points of attachment where ‘X’ shaped structures occur at the sites of crossing over is called Chiasmata. Chiasmata are chromatin structures at sites where recombination has been taken place. They are specialized chromosomal structures that hold the homologous chromosomes together.
   Sister chromatids remain closely associated whereas the homologous chromosomes tend to separate from each other but are held together by chiasmata. This substage may last for days or years depending on the sex and organism. The chromosomes are very actively transcribed in females as the egg stores up materials for use during embryonic development. In animals, the chromosomes have prominent loops called the lampbrush chromosomes.