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You are working in a biotechnology lab with a becterium namely E.coli. How will you cut the nucleotide sequence? explain it. - Botany

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प्रश्न

You are working in a biotechnology lab with a becterium namely E.coli. How will you cut the nucleotide sequence? explain it.

टीपा लिहा

उत्तर

The DNA nucleotide sequence can be cut using Restriction endonucleases (RE). Restriction endonucleases – EcoRI cuts the DNA at GAATTC seqUence, producing sticky ends. CTTAAG

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पाठ 4: Principles and Processes of Biotechnology - Evaluation [पृष्ठ ११८]

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सामाचीर कलवी Botany [English] Class 12 TN Board
पाठ 4 Principles and Processes of Biotechnology
Evaluation | Q 19 | पृष्ठ ११८
सामाचीर कलवी Biology (Botany) [English] Class 12 TN Board
पाठ 4 Principles and Processes of Biotechnology
Evaluation | Q 19 | पृष्ठ ९०

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Vaccination gives either permanent or temporary immunity against a specific pathogen or disease. Traditionally, vaccines were prepared with the help of pathogens. Completely or partially killed pathogens were used as vaccines. However, due to this, there were chances of contracting the disease in case of some persons. Hence, as an alternative, scientists tried to artificially produce vaccines with the help of biotechnology. For this purpose, scientists produced the antigen in the laboratory with the help of genes isolated from the pathogen and used it as a vaccine. Thus, safer vaccines are being produced. Work on production of edible vaccines is in progress and presently, potatoes are being produced with the help of biotechnology. These potatoes are called transgenic potatoes. These potatoes will act against bacteria like Vibrio cholerae, Escherichia coli. Consumption of these raw potatoes generates immunity against pathogens. For the production of such edible vaccines, antigens are produced by isolating the desired gene from human pathogens and injecting it into plants.

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