Question

5' – G ^↓ A A T T C - 3'

3' – C T T A A _↑ G - 5'

1. Name the restriction enzyme that recognises the given specific sequence of bases. What are such sequence of bases referred to as?
2. What are the arrows in the given figure indicating? Write the result obtained thereafter.

Answer 1

1. Restriction enzyme: EcoRI.
   Palindromic sequences.
2. The arrows indicate the restriction sites for EcoRI.
   Result:
   5' - G
   A A T T C - 3'
   3' - C T T A A
   G - 5'