Question

Describe the steps involved in Southern blot hybridization using radiolabeled VNTR as a probe.

Answer 1

1. isolation of DNA,
2. digestion of DNA by restriction endonucleases,
3. separation of DNA fragments by electrophoresis,
4. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon,
5. hybridisation using labelled VNTR probe, and
6. detection of hybridised DNA fragments by autoradiography.