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Question
Describe two vectors less methods of gene transfer used in rDNA technology.
Solution
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplast
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
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