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How does the operon function in the presence of lactose? - Biology (Theory)

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Question

How does the operon function in the presence of lactose?

Answer in Brief

Solution

  1. Lactose induces the lac operon. The regulatory gene is activated when lactose is added to the bacteria's medium.
  2. The inducer binds to the repressor protein, rendering it inactive, allowing transcription of the operon.
  3. Thus, the lac operon is negatively regulated in this case.

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Give one significant contribution to the following scientists:

G. Gamow


Short Answer Question:

Describe the structure of ‘Operon’.


Select the WRONG statement from the following with reference to lac operon.


______ refers to the chemical molecule present in the cytoplasm that inactivates the repressor.


Which of the following is TRUE with reference to Operon?


Match the Column I (Enzymes) with Column II (Functions) and select the correct option.

 

Column I

(Enzymes)

 

Column  II

(Functions)

i. Permease P. Transfers an acetyl group from acetyl CO-A to galactoside
ii. β-galactosi dase Q. Entry of lactose into the cell
iii. Transacetylase R. Digests lactose into galactose and glucose
    S. Aminoacylation

Lactose operon produces enzymes ______.


The wild type E. coli cells are growing in normal medium with glucose. They are transferred to a medium containing only lactose as sugar. Which of the following changes takes place?


Select the two correct statements out of the four (A-D) given below about lac operon.

  1. Glucose or galactose may bind with the repressor and inactivate it.
  2. In the absence of lactose, the repressor binds with the operator region.
  3. The z-gene codes for permease.
  4. This was elucidated by Francois Jacob and Jacque Mono.

Study the schematic representation of the genes involved in the lac operon given below and answer the questions that follow:

p i p o z y a

(a) The active site of enzyme permease present in the cell membrane of a bacterium has been blocked by an inhibitor, how will it affect the lac operon?

(b) The protein produced by the i gene has become abnormal due to unknown reasons. Explain its impact on lactose metabolism stating the reason.

(c) If the nutrient medium for the bacteria contains only galactose; will operon be expressed? Justify your answer.


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