Advertisements
Advertisements
Question
How many sets of primers are required in each cycle of PCR?
Solution
Two sets of primers are required in each cycle of PCR.
APPEARS IN
RELATED QUESTIONS
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Which of the following is an INCORRECT match?
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
DNA fingerprinting refers to ______.
Annealing step of PCR operates at ______ °C.
Name the plant disease caused by Agrobacterium tumefaciens.