Question

1. Identify A and B in the following diagram.
2. What is organogenesis?
3. What is meant by hardening?
   
   

Answer 1

1. A: Explant, B: Callus
2. Organogenesis is the induction of roots and shooting in the callus that finally leads to plantlet development.
3. Plantlets grown in a tissue culture laboratory are transferred to polythene bags with sterilised soil and kept under low light and high humidity conditions for an appropriate period of time. This is referred to as hardening.