Question

Identify the desirable characteristics for a plasmid used in rDNA technology from the following.

1. Ability to multiply and express outside the host in a bioreactor.
2. A highly active promoter.
3. A site at which replication can be initiated.
4. One or more identifiable marker genes.
5. One or more unique restriction sites.

Options

• 1, 3, 4 and 5 only

• 2, 3, 4 and 5 only

• 1, 3 and 4 only

• 2, 3 and 5 only

Answer 1

1, 3 and 4 only

Explanation:

An active promoter site and the origin of replication site, often known as the Ori site, are desired properties for a plasmid used in rDNA technology. The plasmid can replicate in the host thanks to these areas. Marker genes that can be used to recognise the recombinants should exist. Restrictions sites must be present for the intended gene to be inserted.