Advertisements
Advertisements
Question
Illustrate using any one example of transgenic bacteria.
Solution
Human insulin has been prepared by genetic technology. Insulin is made up of two small polypeptide chains, chain 'A' and chain 'B' which are linked to each other by disulphide bonds. In human insulin, a pro-hormone is synthesised which contains C peptide. This peptide 'C' is not found in mature insulin, it gets separated from insulin at the time of maturation. In 1983, two DNA sequences corresponding to chain 'A' and 'B' of human insulin were prepared, which were inserted into the plasmid of E. coli to produce insulin chains. These separately produced chains 'A' and 'B' were taken out and joined to each other by disulphide bonds to prepare human insulin. Insulin is a useful medicine for controlling diabetes. The credit for cloning the insulin gene goes to Dr. Saran Narang of Indian origin. He conducted his experiment in Ottawa, Canada.
RELATED QUESTIONS
Expand the Given and mention one application of each:
PCR
State the role of C peptide in human insulin.
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
Which of the following is a neuropeptide hormone?
Define Antigen and Antibody. Name any two diagnostic kits based upon them.
How is a mature, functional insulin hormone different from its prohormone form?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
