Question

In gel electrophoresis, separated DNA fragments can be visualised with the help of ______.

Options

• ethidium bromide in UV radiation

• acetocarmine in UV radiation

• ethidium bromide in infrared radiation

• acetocarmine in bright blue light

Answer 1

In gel electrophoresis, separated DNA fragments can be visualised with the help of ethidium bromide in UV radiation.

Explanation:

We are unable to view pure DNA fragments in visible light or without staining, so the separated DNA fragments must first be stained with the substance ethidium bromide before being exposed to UV light. When DNA is exposed to UV light on an ethidium bromide stained gel, we typically see bands of vivid orange hue.