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Question
Long Answer Question:
Guess (i) the possible location of DNA on the collected evidence from a crime scene and (ii) the possible sources of DNA.
| Evidence | Possible location of DNA on the evidence | Sources of DNA |
| e.g. Eyeglasses | e.g. Ear pieces | e.g. Sweat, Skin |
| Bottle, Can, Glass | Sides, mouthpiece | __________ |
| __________ | Handle | Sweat, skin, blood |
| Used cigarette | Cigarette butt | ____________ |
| Bite mark | ____________ | Saliva |
| __________ | Surface area | Hair, semen, sweet, urine |
Solution
| Evidence | Possible location of DNA on the evidence | Sources of DNA |
| e.g. Eyeglasses | e.g. Ear pieces | e.g. Sweat, Skin |
| Bottle, Can, Glass | Sides, mouthpiece | Saliva, sweat |
| Door, baseball bat, a similar weapon | Handle | Sweat, skin, blood |
| Used cigarette | Cigarette butt | Saliva |
| Bite mark | Persons skin or clothing |
Saliva |
| Blanket, pillow, bedsheet, dirty laundry | Surface area | Hair, semen, sweat, urine |
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RELATED QUESTIONS
Briefly describe the technique employed in DNA fingerprinting.
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
| Column I | Column II |
| A. Alkali treatment | i. Separation of DNA fragments on gel slab |
| B. Southern blotting | ii. Split DNA fragments into single strands |
| C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
| D. PCR | iv. X-ray photography |
| E. Autoradiography | v. Produce fragments of different sizes |
| F. DNA treated with REN | vi. DNA amplification |
Which of the following process is carried out by restriction endonuclease used in DNA fingerprinting?
Identify the CORRECT sequence of steps involved DNA fingerprinting.
i. Isolation of genomic DNA
ii. Separation by electrophoresis
iii. Denaturation of DNA
iv. Hybridization with probe
v. Fragmenting DNA with restriction enzymes
vi. Southern Blotting
vii. Exposure on film
Identify the CORRECT description of DNA fingerprinting.
Which of the following result is obtained when DNA sample hybridized with DNA probe is kept in contact with X-ray film?
Identify the criterion for movement of DNA fragments on agarose gel during gel electrophoresis.
The process in which sample DNA and probe DNA form double stranded DNA is called ____________.
The technique of DNA fingerprinting was first given by ______.
Which of the following is not true with respect to INA fingerprinting?
- DNA fingerprinting technique was first developed by Alec Jeffrey.
- DNA profiling is a more recent and more sensitive version of DNA fingerprinting.
- DNA fingerprinting was first used in Britain to verify the parentage of an immigrant, who had left the country and then wanted to return
- In DNA fingerprinting, single stranded radioactive DNA is used as probes.
Choose the correct option.
