Question

Match the column I (PCR steps) and Column II (Temperature). Select the correct option.

	Column I (PCR steps)		Column II (Temperature)
i.	Polymerization	P.	40 - 60°C
ii.	Denaturation	Q.	70 - 75°C
iii.	Primer annealing	R.	90 - 98°C
		S.	28 - 37°C

Options

• i - P, ii - R, iii - S

• i - R, ii - S, iii - P

• i - Q, ii - R, iii - P

• i - S, ii - R, iii - Q

Answer 1

i - Q, ii - R, iii - P