Advertisements
Advertisements
Question
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Solution
(i) EcoRI
(ii)
| 5'CAG3' | 5'AATTCTTA3' |
| 3'GTCTTAA 5' | 3'GAAT5' |
(iii) Because they create hydrogen connections with their complementary cut sections, these are known as sticky ends.
APPEARS IN
RELATED QUESTIONS
How has the development of bioreactor helped in biotechnology?
Polymerase chain reaction is most useful in ______.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
The PCR technique is used for ______.
Which vector is mostly used in rDNA technology in plants?
How gene amplification is done?
In vitro amplification of DNA segments is called______.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
