Question

pBR322, used very frequently in cloning experiments as a vector in E. coli is ______. 

Options

• an original bacterial plasmid

• a viral genome

• a modified bacterial plasmid

• a transposon

Answer 1

pBR322, used very frequently in cloning experiments as a vector in E. coli is a transposon. 

Explanation:

One of the earliest commonly used E. coli cloning vectors was the plasmid pBR322. It was developed in 1977 and is a modified bacterial plasmid. Tetracycline and ampicillin resistance genes are present in it, allowing the bacteria to flourish on a certain antibiotic-containing media.