Question

Presence of more than one recognition sites within a vector will lead to ______.

Options

• generation of haf fragment

• generation of one fragment

• generation of several fragments

• None of the above

Answer 1

Presence of more than one recognition sites within a vector will lead to generation of several fragments.

Explanation:

If a vector contains more than one recognition site for a specific restriction endonuclease, the enzyme will recognise each of them and produce a cut, resulting in the generation of multiple fragments.