Question

Study the following statements regarding separation of DNA using agarose gel electrophoresis.

1. Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
2. DNA being negatively charged migrates to cathode.

Options

• Statement ii is correct while statement i is incorrect.

• Both the statements i and ii are correct.

• Statement i is correct while statement ii is incorrect.

• Both the statements i and ii are incorrect.

Answer 1

Both the statements i and ii are incorrect.

Explanation:

Small DNA fragments move quicker than large DNA molecules and so separate more quickly. Negatively charged DNA migrates to the anode.