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Question
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Options
Pi-plasmid
cosmid
Ti-plasmid
None of these
Solution
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is Ti-plasmid.
Explanation:
Plant pathogen Agrobacterium tumefaciens works best as a plant genetic engineer. In plants, it results in the development of crown gall. Ti, a plasmid that causes tumours, is a valuable cloning vector in plants. It can be altered with additional DNA, and the plasmid inserts a DNA fragment that changes healthy plant cells into cancerous ones. The insertion of the Ti plasmid into a plant enables it to build its own nutritional machinery since it contains genes for the synthesis of auxin and the hormone cytokinin.
