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Question
Two healthy green plants were placed in the dark for 24 hours. They were then set up, as shown in the figure, for 4 hours. Then a leaf was taken from each plant and
the chlorophyll was removed from the leaves:
( i) What is the next and final step in this experiment?
( ii ) What would be the results of the final step?
( iii ) Why was it necessary to grease the glass sheet?
( iv ) What hypothesis is being tested in this experiment?
Solution
(i) The leaf is then treated with iodine solution.
(ii) The leaf from first plant will not show starch test while the leaf from second plant will become blue-black showing the presence of starch.
(iii) It is necessary to grease the glass sheet to prevent the entry of air containing CO2.
(iv) The hypothesis that CO2 is necessary for photosynthesis is being tested in this experiment.
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The diagram alongside refers to an experiment in which the apparatus was set up with the light source 10 cm away from the plant. After 15 minutes the number of bubbles evolved per minute from the cut stem was recorded. The light source was moved to 20 cm away from the plant, left for 15 minutes and the number of bubbles evolved per minute was again recorded. The experiment was repeated with the light source at distances of 40, 60, 80 and 100 cm away from the plant. Plot a graph for the results obtained and answer the following questions.
(i) From the graph it seems likely that the rate of bubbling per minute at 50 cm would have been
(a) 2.0 (b) 2.5 (c) 3.0 (d) 3.5
(ii) The gas produced by the plant during the experiment was
(a) air (b) oxygen (c) carbon dioxide (d) nitrogen (e) hydrogen
(iii) The gas collected comes due to the breakdown of
(a) glucose (b) starch (c) water (d) air (e) ATP
(iv) If ice cubes were added to the water, the rate of bubble formation would
(a) Stay the same.
(b) Increase because more water is added.
(c) Decrease because the temperature drops.
(d) Decrease because water freezes.
(e) Cannot tell from the information given.
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(b) Increases because more photosynthesis occurs.
(c) Increases because the gas becomes less soluble.
(d) Decreases because carbon dioxide acts as a limiting factor.
(e) Decreases because respiration decreases.
A potted plant was taken in order to prove a factor necessary for photosynthesis. The potted plant was kept in the dark for 24 hours. One of the leaves was covered with black paper in the centre. The potted plant was then placed in sunlight for a few hours.
Why was the plant placed in the dark before beginning the experiment?
A potted plant was taken in order to prove a factor necessary for photosynthesis. The potted plant was kept in the dark for 24 hours. One of the leaves was covered with black paper in the centre. The potted plant was then placed in sunlight for a few hours.
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(b) Explain the physiological process as mentioned above in (a).
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(d) Why does test-tube B have more bubbles of oxygen?
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(f) Write the overall chemical equation for the above process.
Choose the Odd One Out
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Which of the following is used to remove chlorophyll from the leaves?
Given below is the diagram of an experimental set-up (final stage). Study the same and answer the following questions:
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