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Question
What are the assumptions made during the calculation of net gain of ATP?
Solution
For the theoretical calculation of ATP molecules, various assumptions are made, which are as follows:
- It is assumed that various parts of aerobic respiration, such as glycolysis, the TCA cycle, and ETS, occur in a sequential and orderly pathway.
- NADH produced during the process of glycolysis enters the mitochondria to undergo oxidative phosphorylation.
- The glucose molecule is assumed to be the only substrate, while it is assumed that no other molecule enters the pathway at intermediate stages.
- The intermediates produced during respiration are not utilised in any other process.
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