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Question
When cut by the same restriction endonuclease, the DNA fragments with staicky ends can be joined with any DNA fragment that contains complementary staicky ends into a molecule of recombinant DNA by the enzyme ______
Options
DNA polymerase
exonuclease
DNA ligase
Endonuclease
Solution
When cut by the same restriction endonuclease, the DNA fragments with staicky ends can be joined with any DNA fragment that contains complementary staicky ends into a molecule of recombinant DNA by the enzyme DNA ligase.
Explanation:
DNA ligase is the enzyme that joins the sticky ends of D A helping in joining different DNA fragments. Endonucleases cut DNA at specific locations, exonuclease cut DNA at the ends. and polymerase helps in forming a new polymer of DNA or RNA whose sequence is complementary to the original templet.