Question

Which of the following statements are true about cloning vectors?

1. Vectors are DNA molecules that carry a foreign DNA segment.
2. The replicated DNA is introduced into the host cell.
3. It possesses the origin of replication (Ori).
4. Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
5. Vectors do not require a selectable marker to identify and eliminate non-transformants.

Choose the correct answer from the options given below.

Options

• I and IV only

• I, III and IV only

• I and V only

• I and II only

Answer 1

I, III and IV only

Explanation:

Assertions I, III, and IV about cloning Vectors are correct, and other statements can be modified because Vectors carry a foreign DNA fragment and replicate inside the host cell. To identify and delete non-transformants, vectors require a selectable marker.