Question

Which one of the following bacteria were used by Stanley Cohen and Herbert Boyer to accomplish the construction of the first rDNA?

Options

• Agrobacterium tumefaciens

• Mycobacterium sp.

• E. coli

• Salmonella typhimurium

Answer 1

E. coli

Explanation:

In 1973, Stanley Cohen and Herbert Boyer created the first recombinant DNA (rDNA) by using Escherichia coli. They accomplished this by putting a foreign DNA fragment into a plasmid vector and infecting E. coli cells, which then replicated the recombinant DNA. This revolutionary work signalled the beginning of genetic engineering.