Advertisements
Advertisements
प्रश्न
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
उत्तर
The selective marker is used in the selection of recombinants on the basis of the ability to produce colour in the presence of a chromogenic substrate. β-galactosidase is an enzyme that converts galactose into lactose. In this, a recombinant DNA is inserted within the coding sequence of enzyme, β-galactosidase, which results in inactivation of an enzyme referred to as "insertional inactivation".
The coding sequence for the enzyme β-galactosidase is preferred over antibiotic resistance genes because recombinants can be easily visualized.
APPEARS IN
संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
The genetic material of virus includes:
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A clone is a ______.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a cloning vector?
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
