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प्रश्न
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
उत्तर
The gene for the enzyme β-galactosidase is an alternative selectable marker. When the foreign gene is inserted within the β-galactosidase gene, the enzyme β- galactosidase gets inactivated. Then the bacteria are grown on a chromogenic substrate. Non-recombinants will produce blue-coloured colonies, while recombinants will produce colourless colonies.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Describe briefly the following:
Origin of replication
Write the role of Ori and 'restriction' site in a cloning vector pBR322.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
The genetic material of virus includes:
A clone is a ______.
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
