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प्रश्न
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
उत्तर
The gene for the enzyme β-galactosidase is an alternative selectable marker. When the foreign gene is inserted within the β-galactosidase gene, the enzyme β- galactosidase gets inactivated. Then the bacteria are grown on a chromogenic substrate. Non-recombinants will produce blue-coloured colonies, while recombinants will produce colourless colonies.
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संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Write the role of Ori and 'restriction' site in a cloning vector pBR322.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
