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प्रश्न
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
उत्तर
Agrobacterium tumefaciens harbours a megaplasmid called Ti-plasmid. This has a T-DNA region flanked by the left border and right border sequence. The T-DNA gets transferred and integrates with the host plant DNA. This property of Ti-plasmid has been exploited for cloning gene of interest and stably integrating them into the plant genese. Therefore, by using Ti-plasmid or its derivatives, recombinant plant cells with desired genes of interest stably integrated in the plant genome have been successfully produced.
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संबंधित प्रश्न
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
What is ‘Ori’ ? State its importance during cloning of a vector.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What does ‘competent’ refer to in competent cells used in transformation experiments?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
