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प्रश्न
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
उत्तर
Agrobacterium tumefaciens harbours a megaplasmid called Ti-plasmid. This has a T-DNA region flanked by the left border and right border sequence. The T-DNA gets transferred and integrates with the host plant DNA. This property of Ti-plasmid has been exploited for cloning gene of interest and stably integrating them into the plant genese. Therefore, by using Ti-plasmid or its derivatives, recombinant plant cells with desired genes of interest stably integrated in the plant genome have been successfully produced.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Describe briefly the following:
Origin of replication
Write the role of Ori and 'restriction' site in a cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
A clone is a ______.
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
