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प्रश्न
What is ‘Ori’ ? State its importance during cloning of a vector.
उत्तर
Origin of Replication (Ori):- This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells.
Importance of Ori:-
(i) It is responsible for controlling the copy number & the linked DNA.
(ii) In cloning if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.
APPEARS IN
संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following statement is not true for a clone?
Which of the following is not a genetic vector?
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Describe the role of CaCl2 in the preparation of competent cells?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
