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प्रश्न
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
उत्तर
Importance of Selectable Marker:-
(i) It helps in identifying and eliminating non-transformants.
(ii) It selectively permitting the growth of the transformant.
e.g- The genes encoding resistance to antibiotics such as Ampicillin, Chloramphenicol Tetracyclin or Kanamycin are considered useful selectable markers for E.coli because normally E-coli does not carry resistance against any of these antibiotics.
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संबंधित प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a genetic vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
